在苯甲酰化二乙氨基乙基纤维素柱上纯化肽基转移核糖核酸
Purification of peptidyl-tRNA on benzoylated DEAE-cellulose columns.
作者信息
Eilat D, Ziv E, Lapidot Y
出版信息
Nucleic Acids Res. 1975 Dec;2(12):2237-50. doi: 10.1093/nar/2.12.2237.
Abstract
The o-nitrophenylsulfenyl group, and amino protecting group in the chemical synthesis of peptidyl-tRNA was used to attach newly synthesized peptidyl-tRNA to a benzoylated DEAE-cellulose column. This facilitated the isolation of a highly purified specific tRNA with a well defined peptide chain.
摘要
在肽基 - tRNA的化学合成中，邻硝基苯硫基和氨基保护基团被用于将新合成的肽基 - tRNA连接到苯甲酰化的DEAE - 纤维素柱上。这有助于分离出具有明确肽链的高度纯化的特异性tRNA。
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