Bakst M R
Germplasm and Gamete Physiology Laboratory, Agricultural Research Service-USDA Beltsville, Maryland 20705.
Biol Reprod. 1994 May;50(5):987-92. doi: 10.1095/biolreprod50.5.987.
A novel approach was used to evaluate the distribution of sperm in the oviduct of turkey hens inseminated before or after the onset of egg production. Prior to insemination, sperm were stained with the nuclear fluorescent stain bisbenzimide. Sperm distribution in the sperm storage tubules (SST) of the uterovaginal junction and the infundibular tubular glands was determined by use of simultaneous differential interference contrast and fluorescence microscopy. In hens inseminated and examined prior to the onset of egg production, 94% of the SST contained sperm (21% were filled). In contrast, in hens inseminated initially before the onset of egg production and examined after the onset of lay, only 73% of the SST contained sperm (5% were filled); and in hens inseminated initially after the onset of lay and then examined, 78% of the SST contained sperm (4% were filled). Sperm were sparsely distributed in the infundibular tubular glands. Therefore, lower percentages of filled SST were associated with the onset of egg production, an indication that the sperm storage capacity of the SST is diminished with the onset and continuation of egg production. Physical events associated with the daily ovulatory cycle, such as rotation of the egg mass during shell formation, may both displace sperm residing in the SST and diminish the efficacy of sperm entry into the SST.
采用一种新方法来评估在产蛋开始之前或之后输精的母火鸡输卵管中精子的分布情况。在输精前，精子用核荧光染料双苯甲酰亚胺进行染色。通过同时使用微分干涉对比显微镜和荧光显微镜来确定精子在子宫阴道连接处的精子储存小管（SST）和漏斗状管状腺中的分布。在产蛋开始之前输精并检查的母鸡中，94%的SST含有精子（21%充满精子）。相比之下，在产蛋开始之前首次输精并在产蛋开始之后检查的母鸡中，只有73%的SST含有精子（5%充满精子）；而在产蛋开始之后首次输精然后检查的母鸡中，78%的SST含有精子（4%充满精子）。精子在漏斗状管状腺中分布稀疏。因此，充满精子的SST比例较低与产蛋开始有关，这表明随着产蛋开始和持续，SST的精子储存能力会下降。与每日排卵周期相关的物理事件，如蛋壳形成过程中蛋团的旋转，可能既会使驻留在SST中的精子移位，又会降低精子进入SST的效率。
