Härtig W, Hausen D, Brauer K, Arendt T, Bigl V, Brückner G
Department of Neurochemistry, Paul Flechsig Institute for Brain Research, University of Leipzig, Germany.
Neuroreport. 1994 Jan 31;5(5):573-6. doi: 10.1097/00001756-199401000-00010.
Cytochemical procedures for the combined demonstration of astroglia, microglia, blood vessels and beta-amyloid in the human brain were developed. These multiple label experiments include the first adaptation of the digoxigenin-antidigoxigenin technique to immunocytochemistry by using digoxigenylated antibodies directed against the glial fibrillary acidic protein and its visualization in astrocytes with an antidigoxigenin-peroxidase conjugate and diaminobenzidine as chromogen. Furthermore, the specific labelling of microglial cells was performed with the novel enhanced polymer one-step staining technique, non-interfering with conventional detection systems. The demonstration of spatial relationships between glial and vascular components in normal tissue and their alterations, e.g. in Alzheimer's disease, might provide insights into the time course and localization of pathological events.
已开发出用于在人脑中联合显示星形胶质细胞、小胶质细胞、血管和β-淀粉样蛋白的细胞化学方法。这些多重标记实验包括首次将地高辛配体-抗地高辛配体技术应用于免疫细胞化学，方法是使用针对胶质纤维酸性蛋白的地高辛配体化抗体，并通过抗地高辛配体-过氧化物酶结合物和二氨基联苯胺作为显色剂在星形胶质细胞中进行可视化。此外，小胶质细胞的特异性标记采用了新型增强聚合物一步染色技术，该技术不干扰传统检测系统。正常组织中神经胶质和血管成分之间空间关系的显示及其改变，例如在阿尔茨海默病中的改变，可能为病理事件的时间进程和定位提供见解。
