Zambon A, Bronte V, Di Virgilio F, Hanau S, Steinberg T H, Collavo D, Zanovello P
Institute of Oncology, Inter-University Center for Cancer Research, University of Padua, Italy.
Cell Immunol. 1994 Jul;156(2):458-67. doi: 10.1006/cimm.1994.1190.
It has been proposed that extracellular ATP (ATPo) may function as a cytotoxic molecule in Ca(2+)-independent cell-mediated lysis by activating plasma membrane P2 purinergic receptors. In the present study the involvement of the P2z purinergic receptor in ATPo as well as cell-mediated lysis was investigated by using the J774 mouse macrophage cell line, which expresses this receptor, and a panel of J774-derived mutant cell clones selected for the lack of P2z receptor activity. We confirmed that the P2z receptor in J774 is associated with ATPo-induced colloido-osmotic lysis but not with apoptosis. Furthermore, we observed that the lack or the inhibition of the P2z purinergic receptor does not affect lytic activity mediated by different types of cytotoxic cell populations. These results on the whole indicate that the P2z receptor is involved in cell membrane damage induced by ATPo but not in cell-mediated cytotoxicity.
有人提出，细胞外ATP（ATPo）可能通过激活质膜P2嘌呤能受体，在不依赖Ca（2+）的细胞介导的裂解中作为一种细胞毒性分子发挥作用。在本研究中，通过使用表达该受体的J774小鼠巨噬细胞系以及一组因缺乏P2z受体活性而选择的J774衍生的突变细胞克隆，研究了P2z嘌呤能受体在ATPo以及细胞介导的裂解中的作用。我们证实，J774中的P2z受体与ATPo诱导的胶体渗透裂解有关，但与细胞凋亡无关。此外，我们观察到P2z嘌呤能受体的缺失或抑制并不影响不同类型细胞毒性细胞群体介导的裂解活性。总体而言，这些结果表明，P2z受体参与了ATPo诱导的细胞膜损伤，但不参与细胞介导的细胞毒性。
