Schwarting G A, Drinkwater D, Crandall J E
Shriver Center, Waltham, MA 02254.
Brain Res Dev Brain Res. 1994 Apr 15;78(2):191-200. doi: 10.1016/0165-3806(94)90026-4.
A monoclonal antibody, CC6, reacts with a complex glycolipid whose expression in the rat is restricted to the olfactory system. Structural analysis reveals that the glycolipid contains two alpha-fucose branches; one each at internal and external beta-galactose residues. Immunocytochemistry demonstrates in rat embryos that CC6 glycolipid expression is restricted to a subset of neurons in the vomeronasal organ (VNO) and their corresponding axon projections in the caudal accessory olfactory bulb (AOB). This pattern of expression in the accessory olfactory system is the converse of the pattern revealed by a previously characterized antiglycolipid antibody that reacts with VNO neurons projecting to the rostral AOB. The CC6-reactive glycolipid is also expressed on a subset of neurons in the main olfactory epithelium. Postnatally, axons from these CC6 positive sensory neurons converge to form a limited number of axon bundles running longitudinally through the nerve layer of the main olfactory bulb. These data provide further evidence that groups of vomeronasal and olfactory neurons expressing unique surface molecules project axons that terminate in selected targets in the AOB and OB, respectively.
一种单克隆抗体CC6与一种复合糖脂发生反应，该复合糖脂在大鼠体内的表达仅限于嗅觉系统。结构分析表明，这种糖脂含有两个α-岩藻糖分支，分别位于内部和外部的β-半乳糖残基上。免疫细胞化学显示，在大鼠胚胎中，CC6糖脂的表达仅限于犁鼻器（VNO）中的一部分神经元及其在尾侧副嗅球（AOB）中的相应轴突投射。在副嗅觉系统中的这种表达模式与之前一种已鉴定的抗糖脂抗体所揭示的模式相反，该抗体与投射到吻侧AOB的VNO神经元发生反应。CC6反应性糖脂也在主嗅上皮的一部分神经元上表达。出生后，来自这些CC6阳性感觉神经元的轴突汇聚形成有限数量的轴突束，纵向穿过主嗅球的神经层。这些数据进一步证明，表达独特表面分子的犁鼻器和嗅觉神经元群分别将轴突投射到AOB和OB中的特定靶点。
