Jiang X, Naik M U, Hrabe J, Sacktor T C
Department of Pharmacology, SUNY Health Science Center at Brooklyn 11203.
Brain Res Dev Brain Res. 1994 Apr 15;78(2):291-5. doi: 10.1016/0165-3806(94)90038-8.
Protein kinase C (PKC) is a heterogeneous family of ten or more isoforms which plays an important role in neuronal signal transduction. Isoforms from all subclasses are prominently expressed in the rat hippocampus, as demonstrated by immunoblot with isozyme-specific antisera: Ca(2+)-dependent (alpha, beta I, beta II and gamma), Ca(2+)-independent (delta, epsilon and a newly characterized PKC related to eta) and atypical (zeta). In addition, the zeta isoform is also found as the free, constitutively active catalytic domain, protein kinase M zeta (PKM zeta). Two distinct patterns of expression of PKC isozymes in rat hippocampus are found during development from E18 to P28. PKC zeta, PKM zeta and PKC delta are present at birth and their expression does not increase postnatally. In contrast, the other isoforms are expressed only at low levels at birth and then increase in the first 4 weeks postnatally. These two patterns of expression suggest distinct functions for PKC isozymes during development.
蛋白激酶C（PKC）是一个由十种或更多亚型组成的异质性家族，在神经元信号转导中起重要作用。如用同工酶特异性抗血清进行免疫印迹所证明的，所有亚类的亚型在大鼠海马中均有显著表达：依赖钙的（α、βI、βII和γ）、不依赖钙的（δ、ε和一种新鉴定的与η相关的PKC）和非典型的（ζ）。此外，ζ亚型还以游离的、组成型活性催化结构域的形式存在，即蛋白激酶Mζ（PKMζ）。在从胚胎第18天到出生后第28天的发育过程中，发现大鼠海马中PKC同工酶有两种不同的表达模式。PKCζ、PKMζ和PKCδ在出生时就存在，其表达在出生后不增加。相反，其他亚型在出生时仅低水平表达，然后在出生后的前4周增加。这两种表达模式表明PKC同工酶在发育过程中具有不同的功能。
