用储库模型可视化的酶抑制作用：酶抑制剂的半数抑制浓度（I50）与抑制常数之间的关系。

Enzyme inhibition as visualized with the reservoir model: relationships between I50 and inhibition constant(s) of an enzyme inhibitor.

作者信息

Ribeiro J M, Fontes R, Sillero A

机构信息

Departamento de Bioquímica y Biología Molecular y Genética, Facultad de Medicina, Universidad de Extremadura, Badajoz, Spain.

出版信息

Comput Biol Med. 1994 Mar;24(2):129-44. doi: 10.1016/0010-4825(94)90071-x.

DOI:10.1016/0010-4825(94)90071-x

PMID:8026174

Abstract

In the reservoir model, enzyme activity is simulated by holes in the wall of a reservoir (metabolic pool) containing liquid (substrate) at a certain level (concentration). The holes are computer drawn to reflect the kinetic properties of the enzyme, namely its maximum velocity (hole area) and Km (hole position). The model is here presented as a tool to intuitively visualize the effect of the different types of reversible enzyme inhibitors on the kinetic properties of an enzyme. The relationships between the concentration of an inhibitor producing 50% inhibition of an enzyme reaction (I50) and its inhibition constant(s) are also discussed.

摘要

在储库模型中，酶活性通过一个储库（代谢池）壁上的孔来模拟，该储库含有处于一定水平（浓度）的液体（底物）。这些孔是通过计算机绘制的，以反映酶的动力学特性，即其最大速度（孔面积）和米氏常数（孔位置）。该模型在此作为一种工具呈现，用于直观地可视化不同类型的可逆酶抑制剂对酶动力学特性的影响。还讨论了产生50%酶反应抑制作用的抑制剂浓度（I50）与其抑制常数之间的关系。