Dreznik Z, Meininger T A, Barteau J A, Brocksmith D, Soper N J
Department of Surgery, Washington University School of Medicine, St. Louis, Missouri 63110.
Dig Dis Sci. 1994 Jul;39(7):1511-8. doi: 10.1007/BF02088057.
To determine the effect of ileal oleate on fasting intestinal motility, pairs of duodenal and ileal catheters and bipolar duodenal and jejunal seromuscular electrodes were surgically implanted in six dogs. The ileum was perfused with either normal saline (154 mM NaCl) or oleic acid emulsion (152 mM), while intestinal myoelectric activity was continuously monitored. For transit studies, a bolus of [3H]PEG was injected into the duodenum, and jejunal and ileal alliquots were collected every 15 min for a 6-hr study period. Plasma samples were collected for radioimmunoassays of peptide YY and enteroglucagon. Ileal oleate infusion increased the MMC cycle length and decreased the number of MMCs (P < 0.001) and the myoelectric spike-burst frequency/10 min in the duodenum (P < 0.05). Both duodenal-jejunal (P < 0.05) and duodenal-ileal transit (P < 0.01) were delayed markedly by ileal perfusion with oleic acid emulsion as compared to control studies. Ileal oleate increased plasma levels of peptide YY (P < 0.01) and enteroglucagon (P < 0.01). Ileal perfusion with oleate therefore activated the so-called "ileal brake," diminishing duodenal myoelectric spike bursts and slowing intestinal transit while concurrently increasing plasma levels of peptide YY and enteroglucagon.
为了确定回肠油酸对空腹时肠道运动的影响，在6只犬身上手术植入了成对的十二指肠和回肠导管以及双极十二指肠和空肠浆膜肌层电极。回肠分别灌注生理盐水（154 mM氯化钠）或油酸乳剂（152 mM），同时持续监测肠道肌电活动。在转运研究中，向十二指肠注入一剂[3H]聚乙二醇，在6小时的研究期间，每15分钟收集一次空肠和回肠的等分试样。采集血浆样本用于肽YY和肠高血糖素的放射免疫分析。回肠灌注油酸可增加移行性复合运动（MMC）周期长度，减少MMC的数量（P<0.001），并降低十二指肠肌电峰爆发频率/10分钟（P<0.05）。与对照研究相比，回肠灌注油酸乳剂显著延迟了十二指肠-空肠（P<0.05）和十二指肠-回肠转运（P<0.01）。回肠油酸可提高血浆中肽YY（P<0.01）和肠高血糖素（P<0.01）的水平。因此，回肠灌注油酸激活了所谓的“回肠制动”，减少十二指肠肌电峰爆发，减缓肠道转运，同时增加血浆中肽YY和肠高血糖素的水平。
