Salzet M, Wattez C, Bulet P, Malecha J
Laboratoire de Phylogénie moléculaire des Annélides, ER 87 CNRS, Université des Sciences et Technologies de Lille, Villeneuve d'Ascq, France.
FEBS Lett. 1994 Jul 4;348(1):102-6. doi: 10.1016/0014-5793(94)00581-8.
This paper reports the purification of a novel pro-opiomelanocortin derivative peptide (a gamma-melanocyte stimulating hormone-like (gamma-MSH-like) molecule) from the brain of the leech Theromyzon tessulatum. After reverse-phase HPLC purification, the sequence of the gamma-MSH-like peptide (YVMGHFRWDKFamide) was established by a combination of automated Edman degradation, electrospray mass spectrometry measurement, enzymatic treatment and co-elution experiments in reverse-phase HPLC with synthetic peptides.
本文报道了从欧洲医蛭（Theromyzon tessulatum）大脑中纯化出一种新型促阿片-黑素细胞皮质素原衍生肽（一种γ-促黑素细胞激素样（γ-MSH样）分子）。经过反相高效液相色谱（HPLC）纯化后，通过自动埃德曼降解、电喷雾质谱测量、酶处理以及与合成肽进行反相HPLC共洗脱实验相结合的方法，确定了γ-MSH样肽的序列（YVMGHFRWDKFamide）。
