Albano E, Clot P, Comoglio A, Dianzani M U, Tomasi A
Department of Medical Sciences, University of Turin, Novara, Italy.
FEBS Lett. 1994 Jul 4;348(1):65-9. doi: 10.1016/0014-5793(94)00549-4.
The formation of carbon centered free radicals, identified as methylcarbonyl species, was observed using ESR spectroscopy and the spin trapping agent 4-pyridyl-1-oxide-N-t-butyl nitrone (4-POBN) during the oxidation of acetaldehyde by xanthine oxidase. The reaction was dependent upon the presence of OH. radicals and was inhibited by the addition of superoxide dismutase, catalase or OH. radical scavengers. The generation of methylcarbonyl radicals was associated with a doubling of stable acetaldehyde adducts with serum albumin, and 4-POBN or superoxide dismutase and catalase, completely blocked this effect. Thus, methylcarbonyl radicals contributed to acetaldehyde-mediated protein alkylation which is involved in causing toxic as well as immunological reactions ascribed to acetaldehyde.
在用黄嘌呤氧化酶氧化乙醛的过程中，使用电子自旋共振光谱法和自旋捕捉剂4-吡啶基-1-氧化物-N-叔丁基硝酮（4-POBN）观察到了以碳为中心的自由基（被鉴定为甲基羰基物种）的形成。该反应依赖于羟基自由基的存在，并可被超氧化物歧化酶、过氧化氢酶或羟基自由基清除剂的添加所抑制。甲基羰基自由基的产生与血清白蛋白中稳定的乙醛加合物增加一倍有关，而4-POBN或超氧化物歧化酶和过氧化氢酶可完全阻断这种效应。因此，甲基羰基自由基促成了乙醛介导的蛋白质烷基化，这与乙醛引起的毒性和免疫反应有关。
