Bonner J, Williams J
Department of Ecology and Evolutionary Biology, Princeton University, New Jersey 08544-1033.
Dev Biol. 1994 Jul;164(1):325-7. doi: 10.1006/dbio.1994.1202.
Dictyostelium slugs expressing a dominant inhibitor of the cAMP-dependent protein kinase (PKA) selectively in prestalk cells are insensitive to the environmental signals that normally induce culmination (Harwood et al., 1992a). When such slugs do, eventually, attempt to culminate they are unable to activate stalk-specific gene expression and they become arrested in their development. We show here that they are also about half the size of normal slugs and that they move at about half normal speed, even after their size difference has been taken into account. They are also less accurate in orienting their direction of movement towards the light. Thus, in addition to its role in cellular differentiation, PKA is required for several important aspects of slug behaviour.
在柄细胞中选择性表达环磷酸腺苷（cAMP）依赖性蛋白激酶（PKA）显性抑制剂的盘基网柄菌蛞蝓，对通常诱导 culmination 的环境信号不敏感（Harwood 等人，1992a）。当这种蛞蝓最终试图 culminate 时，它们无法激活柄特异性基因表达，并在发育过程中停滞。我们在此表明，即使考虑到它们的大小差异，它们的大小也只有正常蛞蝓的一半左右，移动速度约为正常速度的一半。它们在将移动方向朝向光线定向时也不太准确。因此，除了在细胞分化中的作用外，PKA 对于蛞蝓行为的几个重要方面也是必需的。
