Bouffard D, Duncan L M, Howard C A, Mihm M C, Byers H R
Department of Pathology, Massachusetts General Hospital, Harvard Medical School, Boston.
Hum Pathol. 1994 Jul;25(7):709-14. doi: 10.1016/0046-8177(94)90305-0.
Studies on melanoma cell lines indicate the expression of actin-binding protein (ABP), a peripheral cytoplasmic protein that crosslinks actin, is important for melanoma cell motility. We used an ABP-specific monoclonal antibody to characterize ABP expression in 18 benign nevi and 28 primary and metastatic malignant melanomas. Heterogeneous expression of ABP staining was observed in metastatic melanoma. No clear differences in ABP staining were identified among compound nevi, dysplastic nevi, and superficial spreading melanoma; however, the lentiginous intraepidermal component of the benign and malignant lesions and the pagetoid cells of superficial spreading malignant melanoma were negative for ABP. In contrast, the nested intraepidermal and dermal components of both benign nevi and primary malignant melanoma were positive. The differential expression of ABP of the lentiginous component as opposed to the intraepidermal nests and pagetoid cells of benign nevi or melanoma may represent a capacity of the nested melanocytes to migrate from the epidermis to the dermis during maturation or invasion. Taken together, the findings support that ABP may be important for cell-cell adhesion during tumorigenesis and may play a role in tumor cell ameboid motility during tissue invasion.
对黑色素瘤细胞系的研究表明，肌动蛋白结合蛋白（ABP）是一种交联肌动蛋白的外周细胞质蛋白，其表达对黑色素瘤细胞的运动性很重要。我们使用一种ABP特异性单克隆抗体来表征18例良性痣和28例原发性及转移性恶性黑色素瘤中ABP的表达。在转移性黑色素瘤中观察到ABP染色的异质性表达。在复合痣、发育异常痣和浅表扩散性黑色素瘤之间未发现ABP染色有明显差异；然而，良性和恶性病变的雀斑样表皮内成分以及浅表扩散性恶性黑色素瘤的派杰样细胞ABP呈阴性。相比之下，良性痣和原发性恶性黑色素瘤的巢状表皮内和真皮成分均为阳性。雀斑样成分与良性痣或黑色素瘤的表皮内巢状结构和派杰样细胞相比，ABP的差异表达可能代表巢状黑素细胞在成熟或侵袭过程中从表皮迁移至真皮的能力。综上所述，这些发现支持ABP在肿瘤发生过程中对细胞间黏附可能很重要，并且可能在组织侵袭过程中肿瘤细胞的阿米巴样运动中发挥作用。
