Kitagawa M, Yoshida S, Ishige I, Minami J, Kuwata T, Tanizawa T, Kamiyama R
Department of Pathology, Faculty of Medicine, Tokyo Medical and Dental University, Japan.
Hum Pathol. 1994 Jul;25(7):723-6. doi: 10.1016/0046-8177(94)90307-7.
Acute megakaryoblastic leukemia (AMKL) manifesting myelofibrosis and tumor formation in the liver with marked increase of reticulin is described. The megakaryoblastic nature of the leukemic cells of the bone marrow and the hepatic tumor nodule was established by positive immunohistochemical stains for CD41a and CD41b on frozen tissue sections. Immunolocalization of the platelet-derived growth factor (PDGF) protein and transforming growth factor (TGF)-beta protein also was demonstrated in the leukemic cells of the bone marrow and the hepatic tumor. Further, the deposition of fibronectin that has been known as the ligand of CD41a molecule and collagen types I and IV were recognized in the extracellular matrix of the bone marrow and the hepatic tumor. These results suggest that specific expression of growth factor proteins by the leukemic cells may selectively regulate the fibrosis of the bone marrow as well as the tumor formation of AMKL. The expression of adhesion molecules and growth factor proteins by the leukemic cells and the deposition of extracellular matrix are discussed in relation to the myelofibrosis as well as the tumor-forming nature of AMKL.
本文描述了表现为骨髓纤维化和肝脏肿瘤形成且网状纤维显著增加的急性巨核细胞白血病（AMKL）。通过对冷冻组织切片进行CD41a和CD41b的免疫组织化学染色阳性，确定了骨髓和肝肿瘤结节中白血病细胞的巨核细胞性质。血小板衍生生长因子（PDGF）蛋白和转化生长因子（TGF）-β蛋白在骨髓和肝肿瘤的白血病细胞中也有免疫定位。此外，在骨髓和肝肿瘤的细胞外基质中识别出了已知为CD41a分子配体的纤连蛋白以及I型和IV型胶原的沉积。这些结果表明，白血病细胞生长因子蛋白的特异性表达可能选择性地调节骨髓纤维化以及AMKL的肿瘤形成。讨论了白血病细胞黏附分子和生长因子蛋白的表达以及细胞外基质的沉积与AMKL的骨髓纤维化和肿瘤形成性质的关系。
