使用哨兵血培养系统分析来自潜在感染的人工关节的标本。
Use of Sentinel blood culture system for analysis of specimens from potentially infected prosthetic joints.
作者信息
Baker S, Fraise A P
机构信息
Microbiology Department, Selly Oak Hospital, Birmingham.
出版信息
J Clin Pathol. 1994 May;47(5):475-6. doi: 10.1136/jcp.47.5.475.
The Sentinel blood culture system was used for the analysis of 657 specimens from infected prosthetic joints and blood cultures (83 from prosthetic joints and 574 from standard blood cultures). The positivity rate was similar for specimens from prosthetic joints and blood cultures (18% compared with 14%). However, there was an unacceptable rate of false positive results with specimens from prosthetic joints (58% compared with 8%). This high false positivity rate was due to (i) prolonged incubation and (ii) the lack of blood in these specimens. It is therefore recommended that the Sentinel system should only be used for the initial seven days of incubation of specimens taken from prosthetic joints. Further incubation should take place in a standard incubator and a terminal subculture performed after 21 days.
采用哨兵血培养系统对657份来自感染人工关节的标本和血培养物（83份来自人工关节，574份来自标准血培养）进行分析。人工关节标本和血培养物的阳性率相似（分别为18%和14%）。然而，人工关节标本的假阳性结果率令人无法接受（58%对比8%）。这种高假阳性率是由于（i）孵育时间延长以及（ii）这些标本中血液含量不足。因此，建议哨兵系统仅用于人工关节标本孵育的最初七天。进一步的孵育应在标准培养箱中进行，并在21天后进行终末传代培养。
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