Prabhakaran S K, Kamble S T
Department of Entomology and Environment Programs, University of Nebraska, Lincoln 68583-0816.
J Econ Entomol. 1994 Jun;87(3):541-5. doi: 10.1093/jee/87.3.541.
Esterases from insecticide-resistant and -susceptible strains of German cockroach, Blattella germanica (L.), were isolated and analyzed for differences in activity. The isozymes E5, E6, E7, and E8 of resistant strains indicated about two- to fivefold increases in activity compared with those of the susceptible strain. These isozymes constituted approximately 60% of the total activity in the resistant strains, whereas they contributed only about 40% activity in the susceptible strain. Subcellular distribution studies showed that most esterase activity is present in the 100,000 x g fraction of the homogenate (cytosolic). Only a small portion of activity was membrane bound. Molecular weights of the esterase isozymes calculated based on their retardation coefficients ranged between 48 and 81 kd.
从德国小蠊（Blattella germanica (L.)）抗杀虫剂品系和敏感品系中分离出酯酶，并分析其活性差异。与敏感品系相比，抗性品系的同工酶E5、E6、E7和E8活性增加了约2至5倍。这些同工酶在抗性品系中约占总活性的60%，而在敏感品系中仅贡献约40%的活性。亚细胞分布研究表明，大部分酯酶活性存在于匀浆的100,000 x g组分（胞质溶胶）中。只有一小部分活性与膜结合。根据其阻滞系数计算的酯酶同工酶分子量在48至81 kd之间。
