紫外线和γ射线照射后的DNA修复。I. 大鼠脾细胞。
DNA repair after UV and gamma irradiation. I. Rat spleen cells.
作者信息
Rocchi P, Perocco P, Grilli S, Arfellini G, Franceschi C, Ferreri A M, Prodi G
出版信息
Arch Sci Biol (Bologna). 1976 Jan-Dec;60(1-4):67-77.
An in vitro microculture was set up to standardize DNA enzymatic repair in rat spleen lymphocytes after exposure to ultraviolet or gamma rays by measuring the tritiated thymidine uptake. A relation between irradiation dose and DNA repair is always observable when cell viability and scheduled DNA synthesis are taken into account. This micromethod utilizing rat spleen cells appears to be a suitable system for such a study since it permits a satisfactory evaluation of DNA repair.
建立了一种体外微培养方法，通过测量氚标记胸腺嘧啶核苷摄取量来标准化大鼠脾脏淋巴细胞在暴露于紫外线或γ射线后的DNA酶促修复。当考虑细胞活力和预定的DNA合成时，总能观察到辐射剂量与DNA修复之间的关系。这种利用大鼠脾细胞的微量方法似乎是进行此类研究的合适系统，因为它能够对DNA修复进行令人满意的评估。
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