Peyron E, Nicolas J F, Réano A, Roche P, Thivolet J, Haftek M, Schmitt D, Péronne C, Banchereau J, Rousset F
Inserm U346, Dermatology Clinic, Edouard Herriot Hospital, Lyon, France.
J Immunol. 1994 Aug 1;153(3):1333-9.
Bullous pemphigoid (BP) is an acquired blistering skin disease associated with the production of IgG autoantibodies to the 230-kDa BP Ag (BPAg1). To better characterize the epitopes of BPAg1, we generated immortalized B cell lines secreting human mAbs (HumAbs) to BPAg1 from two BP patients whose sera reacted with native BPAg1 but not with a recombinant BP55 carboxyl-terminal peptide. Ab-producing B cell lines were established by EBV infection of CD40-activated PBMCs. Three independent clonal lines were obtained that secreted IgG HumAbs, including one IgG1 kappa (BP3) and two distinct IgG4 kappa (BP1 and BP2). These three HumAbs immunoprecipitated BPAg1. Blocking immunofluorescence experiments and phylogenetic studies showed that these Abs recognize different epitopes of BPAg1. This analysis with HumAbs further extends the serologic demonstration of the wide variety of epitopes recognized by BPAg1 autoantibodies which contrasts with the limited number of epitopes recognized by thyroid peroxidase monoclonal autoantibodies.
大疱性类天疱疮（BP）是一种获得性水疱性皮肤病，与针对230-kDa BP抗原（BPAg1）产生IgG自身抗体有关。为了更好地表征BPAg1的表位，我们从两名BP患者中生成了分泌针对BPAg1的人源单克隆抗体（HumAbs）的永生化B细胞系，这两名患者的血清与天然BPAg1反应，但不与重组BP55羧基末端肽反应。通过EBV感染CD40激活的外周血单核细胞（PBMC）建立产生抗体的B细胞系。获得了三个独立的克隆系，它们分泌IgG HumAbs，包括一个IgG1 κ（BP3）和两个不同的IgG4 κ（BP1和BP2）。这三种HumAbs免疫沉淀了BPAg1。阻断免疫荧光实验和系统发育研究表明，这些抗体识别BPAg1的不同表位。用HumAbs进行的这项分析进一步扩展了BPAg1自身抗体识别的多种表位的血清学证明，这与甲状腺过氧化物酶单克隆自身抗体识别的有限数量的表位形成对比。
