Shaked G, Alkan M, Nagauker O, Charuzi I, Levy R
Department of Surgery C, Faculty for Health Sciences, Soroka Medical Center of Kupat Holim, Beer Sheva, Israel.
J Trauma. 1994 Jul;37(1):22-9. doi: 10.1097/00005373-199407000-00006.
The production of free oxygen radicals by polymorphonuclear cells (PMNs) was studied in 25 patients after blunt trauma. Superoxide generation significantly increased immediately after trauma and returned to normal soon after the event. Patients were subsequently divided into two groups: those who developed sepsis and those who did not develop infectious complications. Superoxide production by intact PMNs following stimulation by three different stimulants was initially not different in trauma patients who developed sepsis. Follow-up showed an increase in superoxide production when infection complicated the course of trauma patients. Further studies were performed in a cell-free system containing cell membranes and cytosol from patients or healthy controls. No difference in the production of superoxide was found when membranes from trauma patients or controls were mixed with cytosols from controls. When cytosols from patients were mixed with membranes from controls, a significant increase in superoxide production was observed in the group that developed sepsis. Immunoblotting analysis of two protein components of the cytosolic portion of the NADPH oxidase, p47 and p67, were done. The increase in quantity of p47 correlated with the increase in superoxide production during sepsis, and thus may be the major contributor to the high activity.
对25例钝性创伤患者的多形核白细胞（PMNs）产生游离氧自由基的情况进行了研究。创伤后超氧化物生成立即显著增加，并在事件发生后不久恢复正常。患者随后被分为两组：发生脓毒症的患者和未发生感染并发症的患者。在发生脓毒症的创伤患者中，三种不同刺激物刺激后完整PMNs产生超氧化物的情况最初并无差异。随访显示，当感染使创伤患者病情复杂化时，超氧化物生成增加。在含有患者或健康对照者细胞膜和胞浆的无细胞系统中进行了进一步研究。当创伤患者或对照者的细胞膜与对照者的胞浆混合时，未发现超氧化物生成有差异。当患者的胞浆与对照者的细胞膜混合时，在发生脓毒症的组中观察到超氧化物生成显著增加。对NADPH氧化酶胞浆部分的两种蛋白质成分p47和p67进行了免疫印迹分析。p47数量的增加与脓毒症期间超氧化物生成的增加相关，因此可能是高活性的主要促成因素。
