Ohkuma S, Chen S H, Katsura M, Chen D Z, Kuriyama K
Department of Pharmacology, Kyoto Prefectural University of Medicine, Japan.
Jpn J Pharmacol. 1994 Feb;64(2):125-8. doi: 10.1254/jjp.64.125.
The effect of muscimol on N-methyl-D-aspartate (NMDA)-induced injury of primary cultured cerebral cortical neurons was examined. NMDA induced a dose-dependent leakage of LDH activity, which was significantly inhibited by (+-)-5-methyl-10,11-dihydro-5H-dibenzo-[a,d]cyclopentan-5,10-imine (MK-801). Muscimol significantly reduced the NMDA-induced increase of lactic dehydrogenase (LDH) leakage, and bicuculline abolished this protective effect of muscimol. Similarly, muscimol reduced the NMDA-induced increase in trypan blue staining of the cells, and bicuculline suppressed this inhibitory action of muscimol. These results suggest that GABAA-receptor stimulation exerts a protective action against the neuronal injury induced by NMDA-receptor activation.
研究了蝇蕈醇对原代培养的大脑皮质神经元N-甲基-D-天冬氨酸（NMDA）诱导损伤的影响。NMDA诱导乳酸脱氢酶（LDH）活性呈剂量依赖性渗漏，（±）-5-甲基-10,11-二氢-5H-二苯并[a,d]环戊烯-5,10-亚胺（MK-801）可显著抑制该渗漏。蝇蕈醇显著降低NMDA诱导的乳酸脱氢酶（LDH）渗漏增加，荷包牡丹碱可消除蝇蕈醇的这种保护作用。同样，蝇蕈醇减少了NMDA诱导的细胞锥虫蓝染色增加，荷包牡丹碱抑制了蝇蕈醇的这种抑制作用。这些结果表明，GABAA受体刺激对NMDA受体激活诱导的神经元损伤具有保护作用。
