Meyer J S, He W
Dept. of Pathology, St. Luke's Hospital, Chesterfield, MO 63017.
J Surg Oncol. 1994 Jul;56(3):146-52. doi: 10.1002/jso.2930560304.
The protein p53 is a product of a suppressor oncogene. Mutations occurring in 13-15% of breast carcinomas are associated with p53 stainability within nuclei and progression of the tumor. We determined the extent to which p53 abnormality was associated with proliferation by measuring p53 immunohistochemically with a polyclonal antibody and monoclonal PAb1801 in invasive carcinomas of known S-phase fraction (SPF) assessed histologically by bromodeoxyuridine incorporation. Results with the two antibodies always agreed. One of 20 low, 2/18 midrange, and 9/17 high SPF carcinomas were positive for p53. P53 positivity was also related to other indicators of aggressiveness including size of primary tumor, nuclear and nucleolar size, and estrogen and progesterone receptor content, but relationships between p53 and vascular invasion and lymph node metastasis were not found. We conclude that nuclear p53 accumulation is more closely related to proliferation than to invasion and metastasis, and that it identifies some but not all breast carcinomas with high proliferative indices.
蛋白质p53是一种抑癌基因的产物。在13% - 15%的乳腺癌中发生的突变与细胞核内p53的可染色性及肿瘤进展相关。我们通过用多克隆抗体和单克隆PAb1801进行p53免疫组织化学检测，在已知通过溴脱氧尿苷掺入法组织学评估的S期分数（SPF）的浸润性癌中，测定p53异常与增殖相关的程度。两种抗体的结果始终一致。20例低SPF癌中有1例、18例中SPF癌中有2例、17例高SPF癌中有9例p53呈阳性。p53阳性也与其他侵袭性指标相关，包括原发肿瘤大小、核及核仁大小以及雌激素和孕激素受体含量，但未发现p53与血管侵犯和淋巴结转移之间的关系。我们得出结论，细胞核p53积累与增殖的关系比与侵袭和转移的关系更密切，并且它能识别一些但不是所有具有高增殖指数的乳腺癌。
