Vega J A, Del Valle M, Amenta F
Departamento de Morfologia y Biologia Celular, Universidad de Oviedo, Spain.
Mech Ageing Dev. 1994 Jan;73(1):9-16. doi: 10.1016/0047-6374(94)90033-7.
The present study was designed to assess changes in the expression of cytoskeletal proteins in the cerebellar cortex of rats of different ages using immunohistochemical techniques associated with image analysis. Male Wistar rats of 3 months (young), 12 months (adult) and 24 months (old) were used. Neuronal cytoskeleton was investigated using monoclonal antibodies to phosphorylated neurofilament (NF) proteins of high (H), medium (M) and low+H+M molecular weight (NF triplet). In young and adult rats in which the expression of phosphorylated NF immunostaining was similar, a dark-brown immunoreactivity was observed primarily in axons of the white matter and of basket neurons which surround Purkinje neurons and enter in the molecular layer. In adult rats a NF-H immunoreactivity was sometimes observed within the dendritic tree of Purkinje neurons. A significant decrease in the density of NF immunoreactivity involving the three different subunits investigated was found in the cerebellar cortex of old rats. This suggests that cytoskeletal abnormalities occur not only in senile dementia, but also in the so called 'physiological aging'. The cerebellar cortex may represent an interesting model for evaluating age-dependent changes of cytoskeleton.
本研究旨在运用与图像分析相关的免疫组织化学技术，评估不同年龄大鼠小脑皮质中细胞骨架蛋白表达的变化。选用了3个月龄（幼年）、12个月龄（成年）和24个月龄（老年）的雄性Wistar大鼠。使用针对高分子量（H）、中等分子量（M）和低分子量+H+M（NF三联体）的磷酸化神经丝（NF）蛋白的单克隆抗体来研究神经元细胞骨架。在幼年和成年大鼠中，磷酸化NF免疫染色的表达相似，主要在白质轴突以及围绕浦肯野神经元并进入分子层的篮状神经元轴突中观察到深棕色免疫反应性。在成年大鼠中，有时在浦肯野神经元的树突内观察到NF-H免疫反应性。在老年大鼠的小脑皮质中，发现涉及所研究的三种不同亚基的NF免疫反应性密度显著降低。这表明细胞骨架异常不仅发生在老年痴呆中，也发生在所谓的“生理性衰老”中。小脑皮质可能是评估细胞骨架随年龄变化的一个有趣模型。
