Takeda M, Tanaka S, Kido H, Daikoku S, Oka M, Sakai K, Katunuma N
Division of Enzyme Chemistry, University of Tokushima, Japan.
Neurosci Lett. 1994 Feb 28;168(1-2):57-60. doi: 10.1016/0304-3940(94)90415-4.
Amyloid precursor protein (APP) 695 is remarkably expressed in the brain as compared with APP751 and APP770 which are dominant in other tissues. This study showed that human and bovine adrenal medullae dominantly expressed mRNA of APP695 as do brain nerve cells, while the adrenal cortexes expressed mRNAs of APP751 and APP770 as in other non-neural tissues. In immunohistochemistry, chromaffin cells of young rat adrenal medullae and primary cultured bovine chromaffin cells were significantly stained with a monoclonal antibody (mAb) against the common domain of the amino-terminal side of human APPs. At higher magnification, the immunostained cells revealed that APP was granularly distributed not only in the perikaryon but also in the cell processes. These results suggest that primary cultured chromaffin cells representing the state of adrenal medulla in vivo are a useful model for studying the pathophysiological functions of APPs and the mechanism of processing of APPs as a model of neuronal systems.
与在其他组织中占主导地位的APP751和APP770相比，淀粉样前体蛋白（APP）695在大脑中显著表达。本研究表明，人和牛的肾上腺髓质与脑神经细胞一样，主要表达APP695的mRNA，而肾上腺皮质则如其他非神经组织一样表达APP751和APP770的mRNA。在免疫组织化学中，幼鼠肾上腺髓质的嗜铬细胞和原代培养的牛嗜铬细胞被抗人APPs氨基末端侧共同结构域的单克隆抗体（mAb）显著染色。在高倍镜下，免疫染色的细胞显示APP不仅颗粒状分布于核周，还分布于细胞突起中。这些结果表明，代表体内肾上腺髓质状态的原代培养嗜铬细胞是研究APPs病理生理功能以及作为神经元系统模型的APPs加工机制的有用模型。
