转座子Tn916从粪肠球菌到地衣芽孢杆菌的接合转移。

Conjugal transfer of transposon Tn916 from Enterococcus faecalis to Bacillus licheniformis.

作者信息

Herzog-Velikonja B, Podlesek Z, Grabnar M

机构信息

Institute of Biology, University of Ljubljana, Slovenia.

出版信息

Plasmid. 1994 Mar;31(2):201-6. doi: 10.1006/plas.1994.1021.

DOI:10.1006/plas.1994.1021

PMID:8029327

Abstract

Transposon Tn916 was conjugally transferred from Enterococcus faecalis to Bacillus licheniformis bacterial strains ATCC 10716 and ATCC 9945A by filter and broth matings. The Tn916 transfer frequencies to B. licheniformis ranged from 10(-7) to 10(-5) selecting for tetracycline-resistant (Tcr) transconjugants depending on broth or filter mating. Movement of Tn916 was demonstrated when Tcr B. licheniformis transconjugants were mated with Bacillus subtilis strain W23. Tn916 insertion caused several auxotrophic and bacitracin deficient mutants. Southern blot analyses of HindIII chromosomal digests extracted from Tcr transconjugants showed that the transposon inserted at different sites in the B. licheniformis chromosome and the copy number of Tn916 varied. This approach should be useful for genetic studies of B. licheniformis.

摘要

转座子Tn916通过滤膜和肉汤交配从粪肠球菌接合转移至地衣芽孢杆菌菌株ATCC 10716和ATCC 9945A。根据肉汤或滤膜交配情况，Tn916向地衣芽孢杆菌的转移频率在10⁻⁷至10⁻⁵之间，通过选择四环素抗性（Tcr）转接合子来确定。当Tcr地衣芽孢杆菌转接合子与枯草芽孢杆菌菌株W23交配时，证明了Tn916的移动。Tn916插入导致了几个营养缺陷型和杆菌肽缺陷型突变体。从Tcr转接合子中提取的HindIII染色体消化产物的Southern印迹分析表明，转座子插入地衣芽孢杆菌染色体的不同位点，且Tn916的拷贝数有所不同。这种方法对于地衣芽孢杆菌的遗传学研究应该是有用的。