Young E, Wells P, Holloway S, Weitz J, Hirsh J
Hamilton Civic Hospitals Research Centre, Ontario, Canada.
Thromb Haemost. 1994 Mar;71(3):300-4.
We have compared the non-specific binding of unfractionated heparin (UFH) with that of low molecular weight heparin (LMWH) to plasma proteins both ex-vivo and in-vitro. Non-specific binding to plasma proteins was assessed by comparing the heparin levels measured as anti-factor Xa activity before and after the addition of low affinity heparin, which is essentially devoid of anti-factor Xa activity, in order to displace heparin bound to plasma proteins. For the ex-vivo studies, we compared the recovery of UFH and a LMWH (ardeparin) from the plasma of patients participating in a randomized trial of post-operative venous thrombosis prophylaxis. For the in-vitro studies, we compared the recovery of UFH and 4 different LMWHs when added to the plasma from healthy volunteers and from patients with suspected venous thromboembolic disease. The results indicate that the recovery of LMWH is much less affected by nonspecific binding to plasma proteins both ex-vivo and in-vitro. In addition, there are differences between the LMWHs with respect to their plasma protein-binding.
我们已经在体外和体内比较了普通肝素（UFH）与低分子量肝素（LMWH）对血浆蛋白的非特异性结合。通过比较在加入基本没有抗Xa因子活性的低亲和力肝素前后以抗Xa因子活性测量的肝素水平来评估对血浆蛋白的非特异性结合，以置换与血浆蛋白结合的肝素。对于体外研究，我们比较了参与术后静脉血栓形成预防随机试验患者血浆中UFH和一种LMWH（达肝素）的回收率。对于体内研究，我们比较了将UFH和4种不同的LMWH添加到健康志愿者和疑似静脉血栓栓塞疾病患者的血浆后的回收率。结果表明，LMWH的回收率在体外和体内受与血浆蛋白非特异性结合的影响要小得多。此外，不同的LMWH在血浆蛋白结合方面存在差异。
