蛋白酶K在克氏肉孢子虫孢子囊脱囊用于体外培养和DNA提取中的应用。

Use of proteinase K in the excystation of Sarcocystis cruzi sporocysts for in vitro culture and DNA extraction.

作者信息

Ndiritu W, Cawthorn R J, Kibenge F S

机构信息

Department of Pathology and Microbiology, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, Canada.

出版信息

Vet Parasitol. 1994 Mar;52(1-2):57-60. doi: 10.1016/0304-4017(94)90035-3.

DOI:10.1016/0304-4017(94)90035-3

PMID:8030188

Abstract

Proteinase K was used for the cleaning of Sarcocystis cruzi (Apicomplexa) sporocysts prior to excystation. Bovine pulmonary endothelial cell cultures inoculated with the excysted sporozoites remained free of bacterial contamination for the duration of the experiment and had high yields of merozoites. The excysted sporozoites also yielded genomic DNA that could be labelled efficiently with 32P dATP by the random priming method.

摘要

在脱囊前，使用蛋白酶K对克鲁斯肉孢子虫（顶复门）子孢子进行清洗。接种脱囊后的子孢子的牛肺内皮细胞培养物在实验期间没有细菌污染，并且裂殖子产量很高。脱囊后的子孢子还产生了基因组DNA，该DNA可以通过随机引物法用32P dATP有效标记。