Gilligan K J
Department of Neurology, University of Colorado School of Medicine, Denver 80262.
Virology. 1994 Aug 1;202(2):992-6. doi: 10.1006/viro.1994.1425.
The expression of VZV open reading frame (ORF) 21 during viral replication was examined at the RNA level. Three distinct mRNAs homologous to ORF 21, 3.6, 3.0, and 2.4 kb in size, were detected by Northern blot hybridization. Sequence analysis of eight ORF 21 cDNAs indicated that the corresponding RNAs were 3' coterminal and unspliced. The three largest cDNAs contained the entire ORF 21 coding sequence. Two upstream alternative polyadenylation signals located within ORF 21 were not utilized. Sequence analysis of the site of polyadenylation suggests a virus-specific cis-acting element is present in these transcripts.
在RNA水平上检测了水痘-带状疱疹病毒（VZV）开放阅读框（ORF）21在病毒复制过程中的表达。通过Northern印迹杂交检测到三种与ORF 21同源的不同mRNA，大小分别为3.6、3.0和2.4 kb。对八个ORF 21 cDNA的序列分析表明，相应的RNA是3' 共末端且未剪接的。三个最大的cDNA包含完整的ORF 21编码序列。位于ORF 21内的两个上游可变聚腺苷酸化信号未被利用。聚腺苷酸化位点的序列分析表明，这些转录本中存在病毒特异性顺式作用元件。
