Hong C Y, Lo Y C, Tan F C, Wei Y H, Chen C F
Institute of Traditional Medicine, Yang-Ming Medical College, Taipei, Taiwan.
Am J Chin Med. 1994;22(1):63-70. doi: 10.1142/S0192415X94000085.
We isolated rat heart mitochondria and induced lipid peroxidation with ADP and FeSO4. Oxygen consumption and MDA formation were measured for quantitating the amount of lipid peroxidation. Using these methods, we screened the water extracts of 14 Chinese medicinal herbs for their effect on lipid peroxidation. It was found that Astragalus membranaceus inhibited 42.1 +/- 3.4% of oxygen consumption and 39.8 +/- 3.2% of MDA production at concentration of 2 mg dried herb/ml mitochondrial suspension. At the same concentration, Polygonum multiflorum inhibited 52.1 +/- 7.3% of oxygen consumption and 50.9 +/- 5.3% of MDA production. Other herbs did not inhibit lipid peroxidation to 50% of control at concentration up to 6 mg dried herb/ml mitochondrial suspension. Purification and identification of the active component(s) in Astragalus membranaceus and Polygonum multiflorum as well as their clinical application await further studies.
我们分离了大鼠心脏线粒体，并用ADP和硫酸亚铁诱导脂质过氧化。通过测量耗氧量和丙二醛（MDA）的形成来定量脂质过氧化的量。使用这些方法，我们筛选了14种中药的水提取物对脂质过氧化的影响。结果发现，在黄芪干燥药材浓度为2mg/ml线粒体悬浮液时，其抑制了42.1±3.4%的耗氧量和39.8±3.2%的MDA生成。在相同浓度下，何首乌抑制了52.1±7.3%的耗氧量和50.9±5.3%的MDA生成。在高达6mg干燥药材/ml线粒体悬浮液的浓度下，其他草药对脂质过氧化的抑制作用未达到对照组的50%。黄芪和何首乌中活性成分的纯化、鉴定及其临床应用有待进一步研究。
