Thomas G, Ando T, Verma K, Kagan E
Department of Physiology and Biophysics, Georgetown University Medical Center, Washington, District of Columbia 20007.
Ann N Y Acad Sci. 1994 May 28;725:207-12. doi: 10.1111/j.1749-6632.1994.tb39803.x.
This study has shown, for the first time, that in vitro exposure of rat AMs to either crocidolite (amphibole) or chrysotile (serpentine) asbestos fibers induces the synthesis not only of the O2*- anion, but also of the nitrogen radical, NO*. Furthermore, this asbestos-related effect is enhanced in the presence of interferon-gamma. NO* has been implicated in several pathologic reactions, such as inflammation and immune complex-mediated cell injury. Additionally, NO* may interact with secondary amines to generate nitrosamines, which are potent carcinogens. Our findings could represent a novel type of asbestos-mediated injury, and we propose that the injurious effects of asbestos might be mediated via the interaction of NO* with O2*-, with the generation of ONOO- and other potent toxic free radicals.
本研究首次表明，大鼠肺泡巨噬细胞（AMs）在体外暴露于青石棉（闪石）或温石棉（蛇纹石）纤维时，不仅会诱导超氧阴离子（O2*-）的合成，还会诱导氮自由基（NO*）的合成。此外，在干扰素-γ存在的情况下，这种与石棉相关的效应会增强。NO与多种病理反应有关，如炎症和免疫复合物介导的细胞损伤。此外，NO可能与仲胺相互作用生成亚硝胺，而亚硝胺是强效致癌物。我们的研究结果可能代表了一种新型的石棉介导的损伤，并且我们提出石棉的损伤作用可能是通过NO与O2-相互作用，生成过氧亚硝酸根（ONOO-）和其他强效有毒自由基来介导的。
