Kimura T, Kokubu F, Horikoshi S, Tokunaga H, Imai T, Mita S, Adachi M
First Department of Internal Medicine, Showa University, School of Medicine.
Arerugi. 1994 May;43(5):645-51.
Inflammation of mucosa and epithelial damage the of bronchi are characteristic pathological features of asthma. Infiltration of T lymphocytes and eosinophils followed by their activation and release of cytokines seems to be a contributing process. We studied the interaction of interleukin 2 (IL-2) and interleukin 5 (IL-5), cytokines which act on eosinophils, using human peripheral lymphocytes. We found that cyclosporin A (CyA) inhibited the IL-5 production from human peripheral lymphocytes induced by IL-2 stimulation. Human peripheral lymphocytes were isolated from healthy volunteers and bronchial asthma patients with mild eosinophilia. Lymphocyte cultures stimulated with IL-2 were cultured at 37 degrees C in a humidified atmosphere of 5% CO2 in air. After 72 hours' incubation, the proliferative response of the lymphocytes was examined by [3H]thymidine uptake, and at the same time IL-5 in the supernatant of the culture medium was assayed by ELISA. The lymphocytes proliferated on IL-2 stimulation in all cases; IL-5 was detected in 3 out of 9 healthy volunteer cases and 7 out of 11 asthma patient cases. CyA added at the beginning of incubation inhibited both of these responses in a dose-dependent manner.
支气管黏膜炎症和上皮损伤是哮喘的特征性病理表现。T淋巴细胞和嗜酸性粒细胞浸润，随后它们被激活并释放细胞因子，这似乎是一个促成过程。我们使用人外周血淋巴细胞研究了作用于嗜酸性粒细胞的细胞因子白细胞介素2（IL-2）和白细胞介素5（IL-5）之间的相互作用。我们发现环孢素A（CyA）抑制IL-2刺激诱导的人外周血淋巴细胞产生IL-5。从健康志愿者和轻度嗜酸性粒细胞增多的支气管哮喘患者中分离出人外周血淋巴细胞。用IL-2刺激的淋巴细胞培养物在37℃、5%二氧化碳的湿润空气中培养。孵育72小时后，通过[3H]胸苷摄取检测淋巴细胞的增殖反应，同时用ELISA法检测培养基上清液中的IL-5。在所有情况下，淋巴细胞在IL-2刺激下均增殖；在9例健康志愿者中有3例检测到IL-5，在11例哮喘患者中有7例检测到IL-5。孵育开始时添加的CyA以剂量依赖方式抑制这两种反应。
