Schindler J G, Schindler M M, Herna K, Reisinger E, Burk B, Kuhlmann U, Knaack J, Schmidt B, Lange H
Institut für Normale und Pathologische Physiologie, Philipps-Universität, Marburg/Lahn, Germany.
Eur J Clin Chem Clin Biochem. 1994 Mar;32(3):145-52.
Presented is a flow-through method for continuous ammonium-selective enzymatic monitoring of the artificial kidney by means of a bioelectrochemical urea electrode. The urea is converted in an enzyme membrane by covalently bound urease and the ammonium ions are detected by a Nonactin-PVC-membrane. In addition to detailed data from the oxygen-independent solid-state contact sensor, curves are obtained on-line from the patient during the haemodialysis session. The advantages of the method are described in detail. Furthermore, the urea sensor can be used for measurements in heparinized blood.
