Yokomori K, Lai M M
Howard Hughes Medical Institute, University of Southern California School of Medicine, Los Angeles.
Arch Virol Suppl. 1994;9:461-71. doi: 10.1007/978-3-7091-9326-6_45.
Mouse hepatitis virus (MHV), a murine coronavirus, has been shown to utilize carcinoembryonic antigen (CEA) as the receptor. We have demonstrated that MHV can utilize a different isoform of CEA, which is an alternatively spliced gene product that is expressed in different tissues, as a receptor. Furthermore, the CEA molecules from a resistant mouse strain (SJL) have different sequences and yet serve as functional viral receptors. Thus, MHV can use more than a single type of CEA molecule as the receptor. We have also shown that some mouse cell lines express functional CEA molecules and yet are resistant to infections by certain MHV strains. Biochemical studies of the infected cells indicate that MHV infections in these cell lines are blocked at the steps of virus entry. We conclude that MHV entry requires additional cellular factors other than CEA, the viral receptor. The significance of viral receptors and the additional cellular factors in regulating viral tropism is discussed.
小鼠肝炎病毒（MHV）是一种鼠冠状病毒，已被证明可利用癌胚抗原（CEA）作为受体。我们已经证明，MHV可以利用CEA的一种不同异构体作为受体，该异构体是一种在不同组织中表达的选择性剪接基因产物。此外，来自抗性小鼠品系（SJL）的CEA分子具有不同的序列，但仍可作为功能性病毒受体。因此，MHV可以使用不止一种类型的CEA分子作为受体。我们还表明，一些小鼠细胞系表达功能性CEA分子，但对某些MHV毒株的感染具有抗性。对感染细胞的生化研究表明，这些细胞系中的MHV感染在病毒进入步骤受阻。我们得出结论，MHV进入除了病毒受体CEA之外还需要其他细胞因子。本文讨论了病毒受体和其他细胞因子在调节病毒嗜性方面的意义。
