Connelly T J, el-Hayek R, Sukhareva M, Coronado R
Department of Anesthesiology, University of Wisconsin Medical School, Madison 53706.
Biochem Mol Biol Int. 1994 Mar;32(3):441-8.
L-thyroxine activated the Ca2+ release channel (ryanodine receptor) of skeletal muscle. [3H]ryanodine binding was stimulated by L-thyroxine in a dose dependent manner producing a two-fold increase at 250 microM. The same concentration induced a release of approximately 40% of the 45Ca2+ passively loaded into sarcoplasmic reticulum in 100 msec. Ca2+ release channel activity monitored in planar bilayers increased in the presence of 250 microM L-thyroxine from a control open probability of 0.02 +/- 0.03 to 0.17 +/- 0.12. Thyroid hormones may directly open Ca2+ release channels of skeletal muscle, thus altering intracellular Ca2+ homeostasis.
L-甲状腺素激活了骨骼肌的Ca2+释放通道（兰尼碱受体）。L-甲状腺素以剂量依赖性方式刺激[3H]兰尼碱结合，在250微摩尔时增加了两倍。相同浓度在100毫秒内诱导约40%被动加载到肌浆网中的45Ca2+释放。在平面双层中监测的Ca2+释放通道活性在存在250微摩尔L-甲状腺素时从对照开放概率0.02±0.03增加到0.17±0.12。甲状腺激素可能直接打开骨骼肌的Ca2+释放通道，从而改变细胞内Ca2+稳态。
