Nalini S, Balasubramanian K A
Wellcome Trust Research Laboratory, Department of Gastrointestinal Sciences, Christian Medical College & Hospital Vellore, India.
Biochem Mol Biol Int. 1994 Mar;32(3):449-54.
Human gastric juice was found to contain high levels of thiols as detected by reaction with dithionitrobenzoic acid (Ellman's reagent). Analysis of gastric juice for glutathione and cysteine, the known acid soluble thiols in biological system, by HPLC and calorimetric methods, indicated that they are absent in the gastric juice. Further studies on the nature of thiols in the gastric juice, by Sephadex G-25 gel filtration indicated the presence of both high and low molecular weight protein and peptide associated thiols. Dialysis of the gastric juice retained nearly 70% thiols in non-dialysable fraction. These studies suggest that biologically important low molecular weight thiols, glutathione and cysteine are absent in the human gastric juice and the thiols detected are mainly acid soluble proteins and possibly peptide associated thiols.
通过与二硫代硝基苯甲酸（埃尔曼试剂）反应检测发现，人类胃液中含有高水平的硫醇。采用高效液相色谱法（HPLC）和比色法分析胃液中的谷胱甘肽和半胱氨酸（生物系统中已知的酸溶性硫醇），结果表明胃液中不存在这些物质。通过葡聚糖G - 25凝胶过滤对胃液中硫醇的性质进行进一步研究，结果表明存在高分子量和低分子量的与蛋白质及肽相关的硫醇。对胃液进行透析后，近70%的硫醇保留在不可透析部分。这些研究表明，人类胃液中不存在具有生物学重要性的低分子量硫醇（谷胱甘肽和半胱氨酸），检测到的硫醇主要是酸溶性蛋白质以及可能与肽相关的硫醇。
