Hua Z, Wang H, Chen D, Chen Y, Zhu D
Department of Biochemistry, Nanjing University, P. R. China.
Biochem Mol Biol Int. 1994 Mar;32(3):537-43.
Human granulocyte-macrophage colony stimulating factor (hGM-CSF) was cloned into expression vector pIN III-ompA1 and expressed in Escherichia coli JA221. When supplementation with a minor tRNA(AGA/AGG)Arg encoded by the E. coli argU gene, the expression level of hGM-CSF was raised about 3-4-fold, although there is only one rare AGG codon in hGM-CSF cDNA gene.
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