Lamparczyk H, Zarzycki P K, Nowakowska J
Medical Academy, Faculty of Pharmacy, Gdańsk, Poland.
J Chromatogr A. 1994 May 13;668(2):413-7. doi: 10.1016/0021-9673(94)80133-9.
The influence of mobile phase composition, concentration of beta-cyclodextrin and temperature on the high-performance liquid chromatographic separation of norgestrel was studied. In studies of the effect of temperature on the enantioselectivity of (+/-)-norgestrel, acetonitrile-water (25:75, v/v) modified by the addition of beta-cyclodextrin (14 mM) was applied as the mobile phase. Enantiomers were detected using UV detection at 240 nm. The capacity factors were measured over a wide range of column temperatures from -5 to 70 degrees C.
研究了流动相组成、β-环糊精浓度和温度对炔诺孕酮高效液相色谱分离的影响。在研究温度对(±)-炔诺孕酮对映体选择性的影响时，以添加了β-环糊精（14 mM）的乙腈-水（25:75，v/v）作为流动相。使用240 nm的紫外检测法检测对映体。在-5至70℃的宽柱温范围内测量容量因子。
