Stibilj V, Dermelj M, Byrne A R, Simenc T, Stekar J M
Jozef Stefan Institute, Nuclear Chemistry Department, University of Ljubljana, Slovenia.
J Chromatogr A. 1994 May 13;668(2):449-53. doi: 10.1016/0021-9673(94)80138-X.
In view of the importance of establishing reliable selenium concentration levels in different kinds of feedstuffs, the purpose of this work was to develop optimum experimental conditions for the isolation and GC determination of selenium as its chelate with 4-nitro-1,2-diaminobenzene. It was shown that ignition of the sample in an oxygen flask followed by reduction of Se(VI) to Se(IV) and the formation of 5-nitro-2,1,3-benzoselenadiazole chelate in HCl medium is a relatively rapid procedure giving a low blank value and allowing the determination of selenium in commercial feedstuffs and similar biological samples. The method was validated by the analysis of suitable certified or standard reference materials.
鉴于确定不同种类饲料中可靠的硒浓度水平的重要性，本研究的目的是开发最佳实验条件，用于分离并通过气相色谱法测定与4-硝基-1,2-二氨基苯形成螯合物的硒。结果表明，在氧瓶中燃烧样品，随后将Se(VI)还原为Se(IV)，并在HCl介质中形成5-硝基-2,1,3-苯并硒二唑螯合物，这是一种相对快速的方法，空白值低，可用于测定商业饲料及类似生物样品中的硒。该方法通过分析合适的有证标准物质或标准参考物质进行了验证。
