一种小细胞肺癌细胞系中的纯合缺失，涉及3p21区域，该区域在酵母人工染色体中具有明显的不稳定性。
A homozygous deletion in a small cell lung cancer cell line involving a 3p21 region with a marked instability in yeast artificial chromosomes.
作者信息
Kok K, van den Berg A, Veldhuis P M, van der Veen A Y, Franke M, Schoenmakers E F, Hulsbeek M M, van der Hout A H, de Leij L, van de Ven W
机构信息
Department of Medical Genetics, University of Groningen, The Netherlands.
出版信息
Cancer Res. 1994 Aug 1;54(15):4183-7.
All types of lung carcinoma are characterized by a high frequency of loss of sequences from the short arm of chromosome 3, the smallest region of overlap containing D3F15S2 in band p21. Here we characterize a 440-kilobase segment from this region, which we found homozygously deleted in one of our small cell lung cancer-derived cell lines. The homozygous deletion maps between UBE1L and ZnF16, just centromeric to D3F15S2. Yeast artificial chromosomes with inserts originating from the deleted region are very unstable and readily lose parts of their insert.
所有类型的肺癌都具有3号染色体短臂序列缺失频率高的特征，该短臂是位于p21带中包含D3F15S2的最小重叠区域。在此，我们对该区域的一个440千碱基片段进行了特征分析，我们发现它在我们的一个小细胞肺癌衍生细胞系中发生了纯合缺失。该纯合缺失定位于UBE1L和ZnF16之间，恰好在D3F15S2的着丝粒侧。带有源自缺失区域插入片段的酵母人工染色体非常不稳定，很容易丢失其插入片段的部分序列。
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