Li H G
Beijing Medical University.
Zhonghua Wai Ke Za Zhi. 1993 Dec;31(12):714-6.
We have established a stable PCR system and studied the structural anomalies of RB gene by it in osteosarcoma, previous studies have proved that 13-17 exons of RB gene are the deletion hotpoints. We designed a pair of perimers, RB1, RB2 stretching across 14-16 exons and studied 10 cases of osteosarcoma. No structural anomaly was found in the leucocyte. Two cases (20%) showed no PCR product. This result demonstrates for the first time the deletion of RB gene is present in Chinese osteosarcoma patients.
我们建立了一个稳定的聚合酶链反应（PCR）系统，并通过该系统研究骨肉瘤中RB基因的结构异常。以往的研究证明，RB基因的13 - 17外显子是缺失热点。我们设计了一对跨越14 - 16外显子的引物RB1、RB2，并对10例骨肉瘤进行了研究。在白细胞中未发现结构异常。2例（20%）未出现PCR产物。这一结果首次证明中国骨肉瘤患者中存在RB基因的缺失。
