Trentini W C, Murray R G
Can J Microbiol. 1975 Feb;21(2):164-72. doi: 10.1139/m75-024.
When Caryophanon latum was exposed to egg white lysozyme in isotonic sucrose and observed by phase-contrast microscopy, protoplasts emerged along the length of the trichomes, apparently at sites corresponding to cross septa. Electron microscopy of sections revealed that this enzyme initially attacked the core of the septal peptidoglycan and delamination of septa resulted. The inner densely staining layer of the lateral and polar wall (considered to contain peptidoglycan as the major component) remained intact except for destruction at the advancing tip of partial septa; protoplasts or cell debris could escape from the gaps formed at developing septa. Treatment of intact trichomes with pronase, a lipase - phospholipase C mixture, EDTA, glutaraldehyde, or heat, before exposure to egg white lysozyme did not alter this pattern nor did it render the remaining peptidoglycan more susceptible to attack. The wall material external to the peptidoglycan was solubilized by pronase. The peptidoglycan remaining after lysozyme treatment was not morphologically changed by treatment with pronase. Lysozyme derived from Chalaropsis hydrolyzed incomplete septa initially, while the lateral and polar wall and complete septa were degraded later. Therefore, it is most probable that the inner dense layer does contain the peptidoglycan component and that some biochemical maturation distinguishes the substrate for these enzymes in the lateral wall and septa.
当宽嘉利翁菌在等渗蔗糖中暴露于蛋清溶菌酶并通过相差显微镜观察时，原生质体沿着菌丝体的长度出现，显然是在与横隔相对应的部位。切片的电子显微镜观察显示，这种酶最初攻击横隔肽聚糖的核心，导致横隔分层。除了部分横隔前进尖端处的破坏外，侧壁和极壁的内部致密染色层（被认为主要成分是肽聚糖）保持完整；原生质体或细胞碎片可以从发育中的横隔形成的间隙中逸出。在暴露于蛋清溶菌酶之前，用链霉蛋白酶、脂肪酶-磷脂酶C混合物、乙二胺四乙酸、戊二醛或加热处理完整的菌丝体，既不会改变这种模式，也不会使剩余的肽聚糖更容易受到攻击。肽聚糖外部的壁物质被链霉蛋白酶溶解。溶菌酶处理后剩余的肽聚糖经链霉蛋白酶处理后在形态上没有改变。来自拟青霉的溶菌酶最初水解不完整的横隔，而侧壁和极壁以及完整的横隔随后被降解。因此，最有可能的是，内部致密层确实含有肽聚糖成分，并且一些生化成熟过程区分了侧壁和横隔中这些酶的底物。
