低密度脂蛋白在主动脉内皮细胞介导的氧化过程中F2-异前列腺素的形成。

Formation of F2-isoprostanes during aortic endothelial cell-mediated oxidation of low density lipoprotein.

作者信息

Gopaul N K, Nourooz-Zadeh J, Mallet A I, Anggård E E

机构信息

William Harvey Research Institute, St Bartholomew's Hospital Medical College, London, UK.

出版信息

FEBS Lett. 1994 Jul 18;348(3):297-300. doi: 10.1016/0014-5793(94)00628-8.

DOI:10.1016/0014-5793(94)00628-8

PMID:8034058

Abstract

We investigated the formation of F2-isoprostanes produced by non-enzymatic peroxidation of arachidonic acid during rabbit aortic endothelial cell-mediated oxidation of low density lipoprotein (LDL). Free and total (sum of free and esterified) levels of F2-isoprostanes were measured using a solid-phase extraction procedure and gas chromatography-mass spectrometry. Free levels of F2-isoprostanes in native LDL were 0.06 +/- 0.03 ng/mg protein (n = 4), whereas total levels were 0.28 +/- 0.09 ng/mg protein (n = 4). Both free and total levels of the isoprostanes were found to increase during the oxidation. 8-epi-PGF2 alpha was the major isoprostane formed (free and total concentrations after 24 h, 2.50 +/- 0.24 and 6.42 +/- 1.36 ng/mg protein (n = 4), respectively). The release of F2-isoprostanes during aortic endothelial cell-induced oxidation of LDL could be a contributory factor in the development of atherosclerosis.

摘要

我们研究了兔主动脉内皮细胞介导的低密度脂蛋白（LDL）氧化过程中，由花生四烯酸非酶促过氧化产生的F2-异前列腺素的形成。采用固相萃取法和气相色谱-质谱联用技术测定F2-异前列腺素的游离水平和总水平（游离和酯化形式的总和）。天然LDL中F2-异前列腺素的游离水平为0.06±0.03 ng/mg蛋白质（n = 4），而总水平为0.28±0.09 ng/mg蛋白质（n = 4）。发现氧化过程中异前列腺素的游离水平和总水平均升高。8-表-前列腺素F2α是形成的主要异前列腺素（24小时后的游离和总浓度分别为2.50±0.24和6.42±1.36 ng/mg蛋白质，n = 4）。主动脉内皮细胞诱导LDL氧化过程中F2-异前列腺素的释放可能是动脉粥样硬化发展的一个促成因素。