Sei S, Kleiner D E, Kopp J B, Chandra R, Klotman P E, Yarchoan R, Pizzo P A, Mitsuya H
Medicine Branch, National Cancer Institute, NIH, Bethesda, MD 20892.
J Infect Dis. 1994 Aug;170(2):325-33. doi: 10.1093/infdis/170.2.325.
The amount of human immunodeficiency virus type 1 (HIV-1) in various tissues was investigated by polymerase chain reaction (PCR) in 16 patients with end-stage HIV-1 infection and 7 patients with symptomatic but less advanced disease. During postmortem study of the 16 end-stage patients, HIV-1 DNA was found most often in lymph nodes and the spleen (both 100%), lung (93.8%), and colon (87.5%). Biopsied lymph nodes from the 7 symptomatic patients contained substantially higher copy numbers of HIV-1 RNA and DNA than did peripheral blood mononuclear cells (PBMC). Plasma viral RNA levels correlated significantly with the amount of HIV-1 RNA in PBMC (r2 = .86, P = .0025) but not with the level of viral RNA in lymph nodes in patients with symptomatic HIV-1 infection. These data suggest that although lymph nodes represent the main site for HIV-1 infection and replication, the level of circulating viral burden may not be solely determined by the magnitude of active HIV-1 replication in lymph nodes.
通过聚合酶链反应（PCR）对16例晚期人类免疫缺陷病毒1型（HIV-1）感染者和7例有症状但病情较轻的患者的各种组织中的HIV-1含量进行了研究。在对16例晚期患者的尸检研究中，HIV-1 DNA最常出现在淋巴结和脾脏（均为100%）、肺（93.8%）和结肠（87.5%）中。7例有症状患者的活检淋巴结中HIV-1 RNA和DNA的拷贝数明显高于外周血单个核细胞（PBMC）。血浆病毒RNA水平与PBMC中HIV-1 RNA的量显著相关（r2 = 0.86，P = 0.0025），但与有症状HIV-1感染患者淋巴结中的病毒RNA水平无关。这些数据表明，虽然淋巴结是HIV-1感染和复制的主要部位，但循环病毒载量水平可能不完全由淋巴结中活跃的HIV-1复制量决定。
