Nuovo G J, Becker J, Burk M W, Margiotta M, Fuhrer J, Steigbigel R T
Department of Pathology, SUNY at Stony Brook 11794-8691.
J Acquir Immune Defic Syndr (1988). 1994 Sep;7(9):916-23.
This study determined the in situ detection rate of polymerase chain reaction (PCR)-amplified human immunodeficiency virus type 1 (HIV-1) DNA and RNA in lymph nodes and peripheral blood CD4+ cells in six patients with asymptomatic HIV-1 infection and from six people who died of advanced AIDS. The lymph nodes of patients with asymptomatic infection showed expanded germinal centers where, on average, 20% of the CD21+ dendritic cells contained HIV-1 DNA. From 5 to 80% of the CD4+ cells in these lymph nodes contained HIV-1 DNA, as compared with 1-11% of the CD4+ peripheral blood mononuclear cells. The infection in most cells was latent in the asymptomatic group. In contrast, the lymph nodes of patients with advanced AIDS showed marked depletion of both dendritic and CD4+ cells. The majority of the remaining CD4+ cells in the lymph nodes and blood showed PCR-amplified viral DNA and cDNA sequences suggesting the presence of genomic and multiple spliced transcripts. It is concluded that asymptomatic HIV-1 infection is associated with a wide range of latent to active viral-positive CD4+ lymphocytes and dendritic cells in the lymph nodes. Progression to AIDS is characterized by active viral replication in many of the remaining CD4+ cells in the lymph nodes and blood.
本研究测定了6例无症状HIV-1感染者和6例死于晚期艾滋病患者的淋巴结及外周血CD4+细胞中聚合酶链反应（PCR）扩增的人类免疫缺陷病毒1型（HIV-1）DNA和RNA的原位检测率。无症状感染患者的淋巴结生发中心扩大，平均20%的CD21+树突状细胞含有HIV-1 DNA。这些淋巴结中5%至80%的CD4+细胞含有HIV-1 DNA，而外周血单核细胞中CD4+细胞的这一比例为1%至11%。在无症状组中，大多数细胞中的感染处于潜伏状态。相比之下，晚期艾滋病患者的淋巴结显示树突状细胞和CD4+细胞均明显减少。淋巴结和血液中剩余的大多数CD4+细胞显示出PCR扩增的病毒DNA和cDNA序列，表明存在基因组和多种剪接转录本。结论是，无症状HIV-1感染与淋巴结中广泛的潜伏性至活动性病毒阳性CD4+淋巴细胞和树突状细胞有关。进展至艾滋病的特征是淋巴结和血液中许多剩余的CD4+细胞中存在活跃的病毒复制。
