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锥虫可变表面糖蛋白的单顺反子转录本。

A monocistronic transcript for a trypanosome variant surface glycoprotein.

作者信息

Alarcon C M, Son H J, Hall T, Donelson J E

机构信息

Human Nutrition Program, University of Iowa, Iowa City 52242.

出版信息

Mol Cell Biol. 1994 Aug;14(8):5579-91. doi: 10.1128/mcb.14.8.5579-5591.1994.

DOI:10.1128/mcb.14.8.5579-5591.1994
PMID:8035832
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC359077/
Abstract

Many protein-encoding genes of African trypanosomes are transcribed as large polycistronic pre-mRNAs that are processed into individual mRNAs containing a 5' spliced leader and 3' poly(A). The 45- to 60-kb pre-mRNAs encoding some variant surface glycoproteins (VSGs) contain as many as eight unrelated coding regions. Here we identify the promoter for a metacyclic VSG gene that is expressed without duplication in a bloodstream trypanosome clone. This 70-bp promoter is located 2 kb upstream of the telomere-linked VSG gene and directs the synthesis of a monocistronic VSG pre-mRNA lacking the 5' spliced leader. Its sequence only slightly resembles those of other known trypanosome promoters, but it does cross-hybridize with several related sequences elsewhere in the genome. These results suggest that a new class of trypanosome promoters has been found, whose function is to initiate monocistronic transcription of those VSG genes normally expressed during the metacyclic stage.

摘要

非洲锥虫的许多蛋白质编码基因转录为大型多顺反子前体mRNA，这些前体mRNA被加工成含有5'剪接前导序列和3'聚腺苷酸的单个mRNA。编码某些可变表面糖蛋白（VSG）的45至60kb前体mRNA包含多达八个不相关的编码区。在这里，我们鉴定了一个在血流锥虫克隆中无复制表达的循环后期VSG基因的启动子。这个70bp的启动子位于端粒连接的VSG基因上游2kb处，并指导合成缺乏5'剪接前导序列的单顺反子VSG前体mRNA。其序列与其他已知的锥虫启动子序列仅略有相似，但确实与基因组其他位置的几个相关序列发生交叉杂交。这些结果表明，已发现一类新的锥虫启动子，其功能是启动那些在循环后期正常表达的VSG基因的单顺反子转录。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5590/359077/58ce09224a3d/molcellb00008-0581-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5590/359077/fc26ee65f422/molcellb00008-0573-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5590/359077/249a0114f33c/molcellb00008-0573-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5590/359077/8b89333f71aa/molcellb00008-0576-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5590/359077/5f191a7b9da1/molcellb00008-0580-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5590/359077/c03d625008a3/molcellb00008-0580-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5590/359077/58ce09224a3d/molcellb00008-0581-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5590/359077/fc26ee65f422/molcellb00008-0573-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5590/359077/249a0114f33c/molcellb00008-0573-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5590/359077/8b89333f71aa/molcellb00008-0576-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5590/359077/5f191a7b9da1/molcellb00008-0580-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5590/359077/c03d625008a3/molcellb00008-0580-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5590/359077/58ce09224a3d/molcellb00008-0581-a.jpg

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Genes Dev. 1993 Jun;7(6):996-1007. doi: 10.1101/gad.7.6.996.
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Cell. 1993 Feb 12;72(3):397-406. doi: 10.1016/0092-8674(93)90116-8.
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Genomic and transcriptional linkage of the genes for calmodulin, EF-hand 5 protein, and ubiquitin extension protein 52 in Trypanosoma brucei.
VEX1 Influences mVSG Expression During the Transition to Mammalian Infectivity in .VEX1在向哺乳动物传染性转变过程中影响变异表面糖蛋白（mVSG）的表达 。 （你提供的原文句子不完整，这里是按照合理补充后的内容进行翻译，原句中“in”后面缺少具体内容）
Front Cell Dev Biol. 2022 Apr 5;10:851475. doi: 10.3389/fcell.2022.851475. eCollection 2022.
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African trypanosomes expressing multiple VSGs are rapidly eliminated by the host immune system.表达多种 VSG 的非洲锥虫会被宿主免疫系统迅速清除。
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