[食管腺癌、食管腺癌/阿霉素耐药及食管腺癌/阿霉素逆转耐药细胞系中多药耐药与谷胱甘肽（GSH）的相关性]
[The correlation between multidrug resistance and glutathione (GSH) in EAC, EAC/ADR and EAC/ADR(R) cell lines].
作者信息
Wang Q D, Li G D, Liu M J, Zhang Y, Liu J, Zhang T M
机构信息
Henan Institute of Medical Sciences, Zhengzhou.
出版信息
Yao Xue Xue Bao. 1994;29(1):20-3.
Determination of GSH was performed by a modified fluorometric method. The experiments showed that the GSH content in resistant cell line (EAC/ADR) was 4 fold higher than that in sensitive cell line (EAC), being 1.25 +/- 0.35 microgram/10(6) cells in EAC/ADR and 0.31 +/- 0.12 microgram/10(6) cells in EAC. When the drug treatment was discontinued for 36 weeks, the sensitivity was partially recovered and the GSH level in EAC/ADR(R) cell parallelly decreased. In addition, vincristine, mitomycin C, actinomycin D and VP-16 showed no effect on the GSH content in the EAC/ADR cell.
谷胱甘肽（GSH）的测定采用改良荧光法。实验表明，耐药细胞系（EAC/ADR）中的GSH含量比敏感细胞系（EAC）高4倍，EAC/ADR中为1.25±0.35微克/10⁶个细胞，EAC中为0.31±0.12微克/10⁶个细胞。当药物治疗中断36周时，敏感性部分恢复，EAC/ADR（R）细胞中的GSH水平相应降低。此外，长春新碱、丝裂霉素C、放线菌素D和依托泊苷对EAC/ADR细胞中的GSH含量无影响。
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