Gvozdev V A, Kakpakov V T, Mukhovatova L M, Polukarova L Y, Tarantul V Z
Sov J Dev Biol. 1975 Jan;5(1):29-36.
Ecdysterone (beta-ecdysone) and 2-deoxy-alpha-ecdysone specifically inhibit the growth of established embryonic cells of D. melanogaster. A preparation of alpha-ecdysone is 100 times less active than ecdysterone. The action of ecdysterone is eliminated when it is removed within 24 h, but after 48 h the inhibiting influence of the hormone becomes irreversible. The diploid line, triploid and tetraploid sublines are sensitive to ecdysterone. The diploid subline with a spontaneously arisen translocation of an X-chromosome to an autosome of the third pair is substantially more resistant to ecdysterone. At 24 h after the addition of ecdysterone, there is a 2-5-fold suppression of the synthesis of total RNA; DNA synthesis is lowered to the same degree only after 48 h. The gross synthesis of proteins, measured according to the incorporation of C 14-lysine, was not suppressed even after 48 h.
蜕皮甾酮（β-蜕皮激素）和2-脱氧-α-蜕皮激素能特异性抑制黑腹果蝇已建立的胚胎细胞的生长。α-蜕皮激素制剂的活性比蜕皮甾酮低100倍。蜕皮甾酮在24小时内去除时其作用消失，但48小时后该激素的抑制作用变得不可逆。二倍体系、三倍体和四倍体亚系对蜕皮甾酮敏感。具有X染色体自发易位到第三对常染色体的二倍体亚系对蜕皮甾酮的抗性要强得多。添加蜕皮甾酮24小时后，总RNA的合成受到2至5倍的抑制；DNA合成仅在48小时后才降低到相同程度。根据C14-赖氨酸掺入量测定的蛋白质总合成，即使在48小时后也没有受到抑制。
