Zimmerman M C, Contiliano J H, Parsons J R, Prewett A, Billotti J
Department of Orthopaedics, University of Medicine and Dentistry of New Jersey, New Jersey Medical School, Newark 07103.
Am J Sports Med. 1994 May-Jun;22(3):378-86. doi: 10.1177/036354659402200314.
A study was initiated to examine chemically processed patellar tendon allografts in sheep anterior cruciate ligament repairs, both mechanically and histologically. One group of animals received frozen, untreated allografts, one group received frozen grafts that were processed with a chloroform-methanol solvent extraction technique, and one group received frozen tendons treated with a permeation-enhanced extraction technique. All animals were operated on unilaterally, with the contralateral knee acting as a normal, intact control. Histologic analysis after 2 months of implantation revealed similar enhanced cellular repopulation in both chemically treated ligament allografts compared with the more hypocellular, untreated grafts. At 6 months the chloroform-methanol group demonstrated a more aggressive chronic cellular response with numerous thick-walled vessels relative to the untreated and permeation-enhanced grafts. Mechanical testing after 6 months of implantation showed statistically similar anterior drawer resistance in all grafted knees, yet the two chemically extracted grafts had significantly less stiffness than untreated anterior cruciate ligament grafts. Both treatment groups also tended to be weaker than the untreated allografts. All anterior cruciate ligament reconstructions showed excessive anterior drawer laxity and, regardless of treatment, had lower strength and less stiffness than normal anterior cruciate ligament tissue at the 6-month period.
开展了一项研究，从力学和组织学方面检查经化学处理的髌腱同种异体移植物在绵羊前交叉韧带修复中的情况。一组动物接受冷冻的、未处理的同种异体移植物，一组接受用氯仿 - 甲醇溶剂萃取技术处理的冷冻移植物，还有一组接受用渗透增强萃取技术处理的冷冻肌腱。所有动物均接受单侧手术，对侧膝关节作为正常、完整的对照。植入2个月后的组织学分析显示，与细胞较少的未处理移植物相比，两种化学处理的韧带同种异体移植物中的细胞再填充均有所增强。6个月时，相对于未处理的和渗透增强的移植物，氯仿 - 甲醇组表现出更活跃的慢性细胞反应，有许多厚壁血管。植入6个月后的力学测试表明，所有移植膝关节的前抽屉阻力在统计学上相似，但两种化学萃取的移植物的刚度明显低于未处理的前交叉韧带移植物。两个处理组也往往比未处理的同种异体移植物更弱。所有前交叉韧带重建均显示前抽屉松弛过度，并且在6个月时，无论何种处理，其强度和刚度均低于正常前交叉韧带组织。
