Wawrzeńczyk A, Nałecz K A, Nałecz M J
Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warszawa.
Biochem Biophys Res Commun. 1994 Jul 15;202(1):354-9. doi: 10.1006/bbrc.1994.1935.
An influence of carnitine on acetylcholine synthesis from radiolabeled glucose was monitored in neuroblastoma NB-2a cells. Upon addition of carnitine the distribution of its derivatives was found significantly different than the values published for brain, the level of long-chain acyl derivatives being much higher and reaching 60%. Carnitine itself did not change acetylcholine level. Together with choline (20 microM), carnitine was observed to stimulate (by 36%) acetylcholine synthesis in a synergistic way, which indicated that both substrates could be limiting factors of this process in NB-2a cell line of neuroblastoma.
在神经母细胞瘤NB - 2a细胞中监测了肉碱对由放射性标记葡萄糖合成乙酰胆碱的影响。添加肉碱后，发现其衍生物的分布与已公布的大脑中的值有显著差异，长链酰基衍生物的水平要高得多，达到60%。肉碱本身并未改变乙酰胆碱水平。与胆碱（20微摩尔）一起，观察到肉碱以协同方式刺激（提高36%）乙酰胆碱合成，这表明在神经母细胞瘤的NB - 2a细胞系中，这两种底物可能都是该过程的限制因素。
