Leonardi A, Acquaviva R, Marinaccio M, Liguoro D, Fogolari F, Di Jeso B, Formisano S, Consiglio E
Dipartimento di Biologia e Patologia Cellulare e Molecolare L. Califano, C.N.R. II Facoltà di Medicina e Chirurgia, Napoli, Italy.
Biochem Biophys Res Commun. 1994 Jul 15;202(1):38-43. doi: 10.1006/bbrc.1994.1890.
The presence of dimeric thyroglobulin (19S), after reduction and alkylation, suggests that within thyroglobulin there may be intermolecular cross-links, other than disulphide bridges. However, the nature of these intermolecular cross-links is still unknown. In this study, we show the presence of 3-3' dityrosine bridges in the molecule of bovine thyroglobulin by NMR and fluorescence studies. Also, we evaluated the role of iodination in dityrosine formation in vivo and in vitro.
还原和烷基化后二聚体甲状腺球蛋白（19S）的存在表明，在甲状腺球蛋白内部可能存在除二硫键之外的分子间交联。然而，这些分子间交联的性质仍然未知。在本研究中，我们通过核磁共振和荧光研究表明，牛甲状腺球蛋白分子中存在3-3'二酪氨酸桥。此外，我们评估了碘化在体内和体外二酪氨酸形成中的作用。
