Calvanico N J, Swartz S J
Department of Dermatology, Medical College of Wisconsin, Milwaukee.
J Autoimmun. 1994 Apr;7(2):231-42. doi: 10.1006/jaut.1994.1018.
A component from bovine epidermis has been purified and found to react with sera from patients with pemphigus foliaceus (PF). This component appears to have molecular weight characteristics similar to those of a previously reported component of human and bovine epidermis which was reactive with PF sera, but only detectable by radiolabeling. It is composed of an 80 kD and a 45 kD polypeptide which are thought to be covalently linked. By chromatographic data, the native form appears to be a noncovalently joined dimer of the 80 and 45 kD polypeptides. Amino acid analysis and sequence data indicate that the 80 kD component is not desmoglein (DG) nor any other known protein whose sequence is entered in data banks. Previous studies by Stanley indicate that DG is the major target of autoantibodies in PF sera. Our finding of another epidermal component reactive with PF antibodies raises some questions. It is not yet known if PF anti-DG antibodies cross-react with this new epidermal antigen or if these antibodies constitute separate and non-cross-reacting sets. Both possibilities and their ramifications are discussed.
一种来自牛表皮的成分已被纯化，并发现它能与落叶型天疱疮（PF）患者的血清发生反应。该成分的分子量特征似乎与先前报道的、能与PF血清发生反应但只能通过放射性标记检测到的人及牛表皮成分相似。它由一个80kD和一个45kD的多肽组成，这两个多肽被认为是共价连接的。根据色谱数据，天然形式似乎是80kD和45kD多肽的非共价连接二聚体。氨基酸分析和序列数据表明，80kD成分不是桥粒芯糖蛋白（DG），也不是数据库中已录入序列的任何其他已知蛋白质。斯坦利之前的研究表明，DG是PF血清中自身抗体的主要靶点。我们发现另一种与PF抗体发生反应的表皮成分，这引发了一些问题。目前尚不清楚PF抗DG抗体是否会与这种新的表皮抗原发生交叉反应，或者这些抗体是否构成独立且不发生交叉反应的集合。文中对这两种可能性及其影响进行了讨论。
