Ambrogi Lorenzini C, Baldi E, Bucherelli C, Tassoni G
Behav Brain Res. 1994 Apr 18;61(2):191-6. doi: 10.1016/0166-4328(94)90159-7.
The tetrodotoxin (TTX) functional ablation technique was employed in order to evaluate the temporal coordinates of the rat's nucleus basalis magnocellularis (NBM) involvement in memory trace processing. Under ketamine general anesthesia, TTX (10 ng in 1 microliter saline) was stereotaxically administered to rats, either in one or both NBMs. TTX was injected to different groups of rats, respectively 15 min, 6, 24, 48, 96 h after passive avoidance acquisition testing. The rats underwent retrieval testing 48 h later, i.e. after full recovery from TTX effects. Results show that: (1) monolateral TTX blockade significantly impairs PAR conditioned responding if induced up to 6 h but not 24 h after acquisition testing; (2) bilateral TTX blockade dramatically impairs passive avoidance responding up to a 48-h delay but not 96 h after acquisition testing. The results indicate a very profound involvement of NBM in passive avoidance response consolidation. The experimental evidence is discussed together with previous functional ablation findings concerning amygdala, parabrachial nuclei and neocortex.
采用河豚毒素（TTX）功能消融技术，以评估大鼠大细胞基底核（NBM）参与记忆痕迹处理的时间坐标。在氯胺酮全身麻醉下，将TTX（1微升盐水中含10纳克）立体定向注射到大鼠的一个或两个NBM中。分别在被动回避习得测试后15分钟、6小时、24小时、48小时、96小时，将TTX注射到不同组的大鼠体内。48小时后，即从TTX作用完全恢复后，对大鼠进行记忆提取测试。结果表明：（1）单侧TTX阻断显著损害在习得测试后6小时内（而非24小时后）诱导的被动回避条件反应；（2）双侧TTX阻断在习得测试后长达48小时（而非96小时）显著损害被动回避反应。结果表明NBM在被动回避反应巩固中有着非常重要的作用。结合先前关于杏仁核、臂旁核和新皮层的功能消融研究结果，对实验证据进行了讨论。
